Journal: Poultry Science

Article Title: Screening and characterization of Limosilactobacillus reuteri L16 and its probiotic properties in Hy-Line Brown chicks

doi: 10.1016/j.psj.2026.107024

Figure Lengend Snippet: Evaluation of cell surface properties and safety of LAB. (A) Auto-aggregation ability of LAB after 3, 6, and 24 h of static incubation. Statistical significance was assessed relative to strain L2 at 3 and 24 h and relative to strain L5 at 6 h; (B) Cell surface hydrophobicity of LAB assessed using n-hexadecane, ethyl acetate, and xylene, with statistical significance determined relative to strain L2. (C) Adhesion of LAB strains to ileal tissue, with statistical significance determined relative to strain L2. (D) Hemolytic activity of LAB. Data are shown as mean ± SD ( n = 3). Statistical analyses for panels (A–C) were performed using ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test. Ns indicates no significant difference; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: For the hydrophobicity assay, 3 mL of bacterial suspension was mixed separately with 1 mL of xylene, ethyl acetate (Chronchem, Chengdu, China), or n-hexadecane (Macklin, Shanghai, China).

Techniques: Incubation, Cell Surface Hydrophobicity, Activity Assay